Oluwatobi S Oluwafemi
University of Johannesburg, South Africa
Title: Synthesis of gelatin stabilised high aspect ratio gold nanorods with enhanced biological stability as effective photothermal agent for cancer therapy
Biography
Biography: Oluwatobi S Oluwafemi
Abstract
A major challenge in efficient biological application of near infrared gold nanorods is the surfactant bilayer-induced cytotoxicity. Hence, there is need for the synthesis of biocompatible, non-toxic and stable functionalized gold nanorods. Though the use of gelatin as a passivating agent is a promising material for multifunctional coating, the inherent cytotoxicity, biological stability as well as the photothermal application performance of gelatin coated gold nanorods still need to be investigated before in vivo therapeutic application. In this study, synthesis of gelatin conjugated high aspect ratio gold nanorods (Au-NRs) with enhanced stability in biological system and its application in photothermal tumor ablation is herein reported for the first time. The gelatin shell required for the appropriate coating was optimized and investigated for their stability in culture media and relative cytotoxicity towards KM-Luc/GFP (mouse fibroblast histiocytoma cell line) and FM3A-Luc (breast carcinoma cell line) cancer cell lines. The optimized ratio of the gelatin-coated Au-NRs (0.5:1) exhibited enhanced biological media stability, improved temperature elevation and excellent photostability compared to CTAB and PEG capped gold nanorods. The cellular cytotoxicity and in vitro laser cytotoxicity experiments further demonstrate the effectiveness of the gelatin-coated nanorods in efficiently inhibiting deep-embedded tumor cells proliferation.