Samuel Oluwatobi Oluwafemi
University of Johannesburg, South Africa
Title: Synthesis, antibacterial, cytotoxicity and sensing properties of biopolymer -capped silver nanoparticles
Biography
Biography: Samuel Oluwatobi Oluwafemi
Abstract
In this study we report the antibacterial, cytotoxicity and sensing properties of sugar reduced biopolymer-capped silver nanoparticles (Ag-NPs) synthesized via a completely green method. The synthesis involved the use of water, starch and gelatin, and maltose and dextrose as the solvent, stabilizing, and reducing agents respectively while AgNO3 was used as the silver precursor without the use of any accelerator. The as-synthesised Ag-NPs were characterized using UV-vis absorption spectroscopy, Fourier transform infra-red spectroscopy (FTIR), Raman spectroscopy, X-Ray diffraction analysis (XRD) and High resolution transmission electron Microscopy (HR-TEM). All the as-synthesised Ag-NPs had good antibacterial activities against E. coli and two strains of P. aeruginosa, which are antibiotic sensitive and resistant bacteria. The study also indicated that, the time of reaction did not have any significant effect on the antibacterial activity of the Ag-NPs synthesized in this present study, though they are of different particle size. The cytotoxicity evaluation on Human THP-1 monocyte cell line indicated that the as-synthesised Ag-NPs are less toxic than AgNO3 at lower concentrations (2 µg/ml). Furthermore, the as-synthesised Ag-NPs was found to be very useful for colorimetric detection of hydrogen peroxide (H2O2) an environmental pollutant at lower concentration upto 10−10 M with a linear regression coefficient value of 0.88223.
